By Alexander P. Demchenko (auth.), Alexander P. Demchenko (eds.)
Fluorescence reporter is the major part of any sensing or imaging expertise. Its optimum selection and implementation is essential for expanding the sensitivity, precision, multiplexing energy, and in addition the spectral, temporal, and spatial reso- tion in several tools of study and functional research. as a result, layout of ?uorescence journalists with complex homes is among the most crucial difficulties. during this quantity, best specialists during this ?eld offer complicated wisdom at the layout and houses of ?uorescent dyes. natural dyes have been the ?rst ?uorescent fabrics used for analytical reasons, and we realize that they maintain their prime positions opposed to powerful pageant of recent fabrics – conjugated polymers, semiconductor nanocrystals, and steel chelating complexes. lately, molecular and mobile biology bought a helpful device of natural ?uorophores synt- sized by means of phone equipment and integrated into eco-friendly ?uorescent protein and its analogs. calls for of assorted ?uorescence ideas working in spectral, anisotropy, and time domain names require concentrated layout of ?uorescence newshounds good tailored to those thoughts. Near-IR spectral diversity turns into progressively more appealing for numerous purposes, and new dyes emitting during this variety are strongly asked. Two-photonic ?uorescence has develop into one of many significant instruments in bioimaging, and ?uorescence newshounds good tailored to this system are in pressing want. those difficulties can't be solved with out the data of primary rules of dye layout and of actual phenomena at the back of their ?uorescence response.
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Additional resources for Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design
Xu H, Wu HP, Huang F, Song SP, Li WX, Cao Y, Fan CH (2005) Magnetically assisted DNA assays: high selectivity using conjugated polymers for amplified fluorescent transduction. Nucleic Acids Res 33:e83 32. Johansson MK, Cook RM (2003) Intramolecular dimers: a new design strategy for fluorescence-quenched probes. Chemistry 9:3466–3471 33. Tramier M, Coppey-Moisan M (2008) Fluorescence anisotropy imaging microscopy for homo-FRET in living cells. Methods Cell Biol 85:395–414 34. Jares-Erijman EA, Jovin TM (2003) FRET imaging.
In: Demchenko AP (ed) Advanced Fluorescence Reporters in Chemistry and Biology II. Springer Ser Fluoresc 9:41–105 11. Vogt RFJ, Marti GE, Zenger V (2008) Quantitative fluorescence calibration: a tool for assessing the quality of data obtained by fluorescence measurements. In: Resch-Genger U (ed) Standardization and quality assurance in fluorescence measurements I: Springer Ser Fluoresc 5:3–31 12. Demchenko AP (2005) The problem of self-calibration of fluorescence signal in microscale sensor systems.
49 7 Concluding Remarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . T. P. T. Gonc¸alves 1 Introduction Over the last years, fluorescent molecules have been widely used as biomolecular labels, enzyme substrates, environmental indicators, and cellular stains, and thus constitute indispensable tools in chemistry, physics, biology, and medicinal sciences [1–10].
Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design by Alexander P. Demchenko (auth.), Alexander P. Demchenko (eds.)